Li Y, Wu Q, Huang L, Yuan C, Wang J, Yang Q. From the field to the lab-an european view on the global spread of PEDV. doi: 10.1016/j.virusres.2016.05.023.Ĭhoudhury B, Dastjerdi A, Doyle N, Frossard JP, Steinbach F. Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains. Surveillance for PEDV now and in the future and improvements derived from mutant strain vaccines are highly warranted.ĬH/HLJJS/2022 Evolutionary analysis Neutralizing epitope mutations Porcine epidemic diarrhea virus Typing characteristics. PEDV is breaking through original cognitions and moving on a more complex path. The newly isolated prevalent strain CH/HLJJS/2022 belonged to the GII subtype, and thirteen mutations different from other strains were found, including mutations in the highly conserved m and N proteins, and in the S1° and COE neutralizing epitopes of the S protein. In addition, typing markers exist during strain evolution, but isolates are using the fusion of specific amino acids from multiple variant strains to add additional features, as also demonstrated by protein alignments and 3D models of numerous subtype strains. Three of the mutations alter the S-protein spatial structure. For the first time, simultaneous mutations of four amino acids were found in the highly conserved membrane (M) and nucleocapsid (N) proteins, as well as eight amino acid mutations that differed from the vast majority of strains in the spike (S) protein. A genome-wide based phylogenetic analysis suggests that it belongs to the GII subtype, and 96.1-98.9% homology existed in the whole genomes of other strains. Additionally, TMHMM was used for protein function prediction.Ī PEDV virulent strain CH/HLJJS/2022 was successfully isolated in China. Mutant amino acids were aligned using DNAMAN and modeled by SWISS-MODEL, Phyre2 and FirstGlance in JMOL for protein tertiary structure simulation. Nucleotide similarity was calculated using MEGA to construct a phylogenetic tree and DNASTAR. Reverse transcription polymerase chain reaction and immunofluorescence were used to determine the etiology, and the full-length PEDV genome was sequenced. We obtained samples from a Chinese diarrheal swine farm in 2022. This study traced the molecular characteristics of the most recent isolates in China and aimed to provide a basis for the prevention and treatment of PEDV. However, vaccines do not provide sufficient protection against currently circulating strains due to viral mutations. epidemic diarrhea virus (PEDV) variant strains cause great economic losses to the global swine industry. 6 Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. 5 Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, People's Republic of China. 4 College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. 3 Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, People's Republic of China. Ltd., Beijng, 100070, People's Republic of China. 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
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